LOCUS       40924_2617        4635 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-BSD2 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Koshimizu S, Aono N, Sasaki-Sekimoto Y, Shigenobu S, Shimojima M, 
            Ohta H, Kofuji R, Tamada Y, Murata T, Hasebe M.
  TITLE     Physcomitrella MADS-box genes regulate external water conduction and
            sperm movement necessary for fertilization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Hiwatashi Y, Koshimizu S, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2017) Contact:Shizuka Koshimizu National Institute
            for Basic Biology, Division of Evolutionary Biology; Nishigonaka 38,
            Myodaiji, Okazaki, Aichi 444-8585, Japan URL 
            :http://www.nibb.ac.jp/evodevo/
REFERENCE   3  (bases 1 to 4635)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2017) Contact:Shizuka Koshimizu National Institute for Basic
            Biology, Division of Evolutionary Biology; Nishigonaka 38, Myodaiji,
            Okazaki, Aichi 444-8585, Japan URL :http://www.nibb.ac.jp/evodevo/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        766..1111
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1150..1536
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1682..1858
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2394..2410)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2447..2465)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2486..2502)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2510..2526)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2534..2564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2579..2600)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2888..3476)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3650..4507)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4508..4612)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agccccccta
      721 ctccaaaaat gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca
      781 aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
      841 aaggacagta gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
      901 tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
      961 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat
     1021 ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat
     1081 ataaggaagt tcatttcatt tggagaggac agcccaagca aggaggtgag ctttctcatt
     1141 gtcaatatgc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     1201 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
     1261 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     1321 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     1381 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     1441 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     1501 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag caccagccat tgatcttgta
     1561 cattcaagct actttctgtt actagatgaa ccgagcatga gttgatggtg acgcgggact
     1621 ctggggttcg gacggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta
     1681 ttttctccat aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg
     1741 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
     1801 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc
     1861 ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga cacgagacga ctaaacctgg
     1921 agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga
     1981 tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt
     2041 ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga
     2101 agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag ggaatgcttg
     2161 tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc
     2221 ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat
     2281 aaaacctcac caaaatacga aagagttctt aactctaaag ataaaagatc tttcaagatc
     2341 aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg atatctcgag ggggatccac
     2401 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     2461 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2521 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2581 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2641 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2701 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2761 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2821 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2881 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2941 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3001 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3061 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3121 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3181 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3241 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3301 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3361 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3421 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3481 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3541 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3601 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3661 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3721 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3781 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3841 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3901 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3961 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4021 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4081 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4141 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4201 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4261 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4321 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4381 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4441 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4501 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4561 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4621 cccgaaaagt gccac
//
